Here are the amounts of the reagents which you will need in order to make 50 L of the second formulation:
– You will need 37575 mL of the 99.8 percent isopropyl alcohol.
– You will need 2085 mL of the 3 percent hydrogen peroxide.
– You will need 725 mL of the 98 percent glycerol.
– You will need enough sterile distilled water or boiled cold water in order to fill the remainder of the bottle to the 50 L mark (approximately 9615 mL of water).
Step by Step Guide to the Preparation of 50 L of Formulation 2
You will first need to pour the 99.8 percent isopropyl alcohol into either the 50 L plastic tank or the 80 to 100 L capacity stainless steel tank. If the opening of your mixing container has a small mouth, avoid spilling any of the ingredients by placing the metal or plastic funnel in the opening of the container to ensure that none of the ingredients spill or splash out of the mixing container. However, if the opening of your mixing container is quite large, the funnel will be neither necessary nor useful. Just be careful when pouring your ingredients into the tank to make sure they do not splash out of the container and make a mess.
You will then need to add the 3 percent hydrogen peroxide into the plastic or stainless-steel tank by using the measuring cylinder. Again, you will need to use the funnel only if your mixing container has a small opening.
Next, you will need to add the 98 percent glycerol into the plastic or stainless-steel tank with the use of a measuring cylinder. Because glycerol is very sticky and viscous and will stick to the glass wall of the measuring cylinder, the cylinder ought to be rinsed with some of that sterile distilled water or cold boiled water and emptied again into the tank.
You will then top up the plastic or stainless-steel tank with the sterile distilled water or the cold boiled water to the 50 L mark.
Now, place the screw-top cap or the lid on the plastic or stainless-steel tank to close it as soon after preparation as possible, in order to prevent the solution from evaporating.
You will then mix the solution within your plastic or stainless-steel tank by using a metal, wooden, or plastic paddle. Do not use an electric mixer, because this may cause the mixture to explode.
You should then divide the solution up into the final containers in which it will reside. For instance, if you intend to carry some hand sanitizer around with you, make sure that you have appropriately sized bottles for travel use.
Place the bottles which are serving as the final containers of your hand sanitizer in quarantine for 72 hours before you plan to use them. This period will allow for any bacterial spores which are present in either the alcohol or the bottles to be destroyed by the hydrogen peroxide. Whenever you distribute the hand sanitizer into its final containers, those containers should be placed in quarantine for 72 hours before you use the hand sanitizer contained within them.