Here are the amounts of the reagents which you will need in order to make 5 L of the second formulation:
– You will need 3757.5 mL of the 99.8 percent isopropyl alcohol.
– You will need 208.5 mL of the 3 percent hydrogen peroxide.
– You will need 72.5 mL of the 98 percent glycerol.
– You will need enough sterile distilled water or boiled cold water in order to fill the remainder of the bottle to the 5 L mark (approximately 961.5 mL of water).
Step by Step Guide to the Preparation of 5 L of Formulation 2
You will first need to pour the 99.8 percent isopropyl alcohol into the 5 L plastic or glass bottle with the stopper that has screw threading. Do this by placing the metal or plastic funnel on top of the bottle to ensure that none of the ingredients spill or splash out of the mixing container.
You will then need to add the 3 percent hydrogen peroxide into the 5 L bottle by using the measuring cylinder. Again, use the funnel to ensure that the addition of this product does not get messy.
Next, you will need to add the 98 percent glycerol into the 5 L bottle with the use of a measuring cylinder. Because glycerol is very sticky and viscous and will stick to the glass wall of the measuring cylinder, the cylinder ought to be rinsed with some of that sterile distilled water or cold boiled water and emptied again into the 5 L bottle.
You will then top up the 5 L bottle with the sterile distilled water or the cold boiled water to the 5 L mark.
Now, place the stopper with screw threading on the 5 L bottle to close it as soon after preparation as possible, in order to prevent the solution from evaporating.
You will then mix the solution within your closed 5 L bottle by either shaking it gently (if this is appropriate) or by using a metal, wooden, or plastic paddle to mix it. Do not use an electric mixer, because this may cause the mixture to explode.
You should then divide the solution up into the final containers in which it will reside. For instance, if you intend to carry some hand sanitizer around with you, make sure that you have appropriately sized bottles for travel use.
Place the bottles which are serving as the final containers of your hand sanitizer in quarantine for 72 hours before you plan to use them. This period will allow for any bacterial spores which are present in either the alcohol or the bottles to be destroyed by the hydrogen peroxide. Whenever you distribute the hand sanitizer into its final containers, those containers should be placed in quarantine for 72 hours before you use the hand sanitizer contained within them.